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Technical Data for CyBi ®-Cellight
Technical Data of CyBi ®-Cellight consist of the data of the following instruments.
Technical Data for CyBi ®-Lumax flash
| CyBi®-Lumax flash HS | CyBi®-Lumax flash HT | | Ultimate sensitivity ...achieved by the use of a proprietary lens with a high optical aperture and excellent photon collection efficiency (CyBi®-Luminox lens), and the beneficial reproduction scale. | Ultra high throughput ...achieved by the use of a 16-channel dispensing head and a detection technology that measures light emitted from all wells of a plate at the same time. | | Detection modes | Flash luminescence
Glow luminescence | Flash luminescence
Glow luminescence | | Detector | Image intensifer plus CCD camera, 2-D single photon counting CyBi®-Luminox lens | Image intensifer plus CCD camera, 2-D single photon counting Leica® lens | | Detection Limit | 60 fg / well Aequorin
200 fg / well Luciferase | 0.3 pg / well Aequorin
1.0 pg / well Luciferase | | Dispenser | Two 8-channel non-contact dispensers, optimized for cells, with cell recirculation | Two 16-channel non-contact dispensers, optimized for cells, with cell recirculation; optional 8-channel heads for 96-well plates | | Volume Range | 0.5 µL – 50 µL | 2 µL – 25 µL | | Plate Formats | 384- and 1536-well | 96-, 384- and 1536-well | | Reading Time | 384: 5.5 minutes
1536: 11.0 minutes | 384: 1.0 minutes
1536: 3.0 minutes |
Technical Data for CyBi ®-Well
Channels,
fixed heads | 96- or 384-channel manifold heads for disposable tips
96/25 µL shallow well
96/25 µL deep well
96/250 µL deep well
384/25 µL shallow well | Tips (µL) | 250 deep well
250 shallow well
60; 25; 10 polypropylene PP disposable tips in standard-, sterile-, PCR-certified or APR compatible quality | Volume range/
working range | Wet pipetting: 200 nL - 250 µL
Dry pipetting: 200 nL - 250 µL
(measured by fluorescence readout) | | Volume resolution | Head 96/250 0.1 µL
Heads 96/25; 384/25 0.01 µL | | Plate formats | shallow well and deep well microplates: 96; 384; 1536 | | Plate positions | 4; 5 | Dimensions
w/h/d (mm) | 1300 / 501- 542 / 260 (4-place carriage)
shallow well or deep well respectively | Weight | Approx. 18.5 kg Shallowwell type | | Operating conditions | +15 - +35 °C; humidity less than 75% at 35°C |
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