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Nucleic Acids

One of the most widespread applications in molecular biology is the purification of nucleic acids from various sources. These include plasmid purification from bacterial and cell cultures, genomic and viral DNA and RNA extraction from blood or other body fluids, cells and animal and plant tissue, purification of PCR products and squencing reaction dye terminator removal. CyBio offers a range of solutions to automate these applications, e.g. the CyBi-WellFlex, the CyBi-WellFlex vario and the CyBi-InLine 8+1/250 l.

The CyBi-Diluspro is a dedicated instrument for nucleic acid purification with magnetic beads in shallow well plates. For magnetic bead separations or for vacuum-based nucleic acid purifications, the CyBi-RoboSpense is equipped. For PCR and sequencing reaction setup the CyBi-Diluspro and the CyBi-RoboSpense can be used. For more complex applications such as DNA normalization after purification procedures, clone picking and reformatting applications or sample specific primer addition prior to PCR reactions, the flexible CyBi-RoboSpense is a great choice.

Application and Technical Notes
Semi-automated genomic DNA extraction from Plant with CyBi-SELMA 96/1000 μl using NucleoSpin 96 Plant II Kit
Automated Quantitive Real Time PCR Set-Up Using the CyBi-8plus1 Automated PCR set-up using the 8-channel pipettor CyBi®-DiluSpro Semi-automated PCR product purification with the CyBi®-DiluSpro using MACHEREY-NAGEL NucleoFast® 96 PCR system
Genomic DNA
Automation of Genomic DNA Purification from Blood with the CyBi®-RoboSpense Using the MACHEREY-NAGEL NucleoSpin® 8/96 Blood Kit Automated Pipetting of a Restriction Digestion with the 8-Channel Pipettor CyBi®-DiluSpro Fully Automated Extraction of Total RNA from Serum and Detection of PRRS Virus Using a CyBi®-RoboSpense Fully automated genomic DNA extraction from wheat with CyBi®-RoboSpense using MACHEREY-NAGEL NucleoMag 96 Plant Kit Isolation and detection of Mycobacterium avium ssp. paratuberculosis in milk samples as automated on a CyBi®-RoboSpense Automated Genomic DNA Extraction from Plants with the CyBi®-RoboSpense using the MACHEREY-NAGEL NucleoSpin® 96 Plant Kit Automation of Genomic DNA Purification from Various Sample Materials with the CyBi®-RoboSpense using the MACHEREY-NAGEL NucleoSpin® 96 Tissue Kit
RNA interference
Automation of Total RNA Purification from HeLa Cells and Mouse Liver Tissue with the CyBi®-RoboSpense Using MACHEREY-NAGEL NucleoSpin® 96 RNA Kit How to Perform High Throughput siRNA Transfections RRR-System - Rapid move to high troughput of siRNA
Sequencing Reaction
Automated sequencing reaction set-up with the CyBi®-DiluSpro using BigDye®Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems)
Automated Plasmid Purification with the CyBi®-RoboSpense Using  MACHEREY-NAGEL NucleoSpin® Robot-96 Plasmid Kit
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